Phylogenetic analysis of plasmid pAKD4— To confirm that pAKD4 is an IncP-1 δ plasmid, a maximum likelihood tree was inferred from concatenated sequences of 20 backbone proteins shared by all ...
Semantic Scholar extracted view of "Comparative genomics of pAKD4, the prototype IncP-1delta plasmid with a complete backbone." by Diya Sen et al.
Plasmid pAKD4 was previously isolated by exogenous plasmid isolation from an agricultural soil in Norway. Its 56,803bp nucleotide sequence shows high similarity in gene sequence and gene order to both plasmids pEST4011 and pIJB1, the only other IncP-1delta plasmids sequenced so far.
These plasmids are clearly distinct from the IncP-1 ␦ plasmid pAKD4, which was isolated from one of the three soils in the same study and described previously (27).
To improve our insight into the evolutionary history of IncP-1 plasmids, the complete nucleotide sequence of the circular plasmid pAKD4 was analyzed. General sequence features of plasmid pAKD4 revealed the following: the genome is 56,803 bp long and its G + C content is 62.7%, which is similar to other IncP-1 plasmids.
To further improve our insight into the diversity and evolutionary history of this family of broad-host-range plasmids, we compared the complete nucleotide sequence of a new IncP-1δ plasmid pAKD4 to the genomes of other IncP-1 plasmids.