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Is the Novel Slot Blot a Useful Method for Quantification of ...

B Slot-Blot Hybridization Analysis. One approach to analyzing a large number of RNA samples in a single experiment is by slot-blot analysis (Green and Sambrook, 2012). In this method, denatured total or poly(A)-enriched RNA is applied to a solid support (typically a nitrocellulose membrane) with the aid of a vacuum applied through slots in a ...

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