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Hydrophobic coated FP3S500 Slot Pins transferred less DMSO - DNA and less Tris DNA and less Tris BSA than the uncoated FP3S500 Slot Pins. 5. Both coated and uncoated FP3S500 pins transfer significantly more aqueous solution than DMSO. ... Note: Same volume (200ul for 96 Format and 74 ul for 384 Format) in recipient plates and same pin ...

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Simply replace the tris buffer in the resolving gel with the Bis-Tris buffer at pH 6.4 (or whatever pH you need so long as it's within the aforementioned buffering range). You can use a lower concentration of Bis-Tris though compared to the concentration of tris you would normally use. Around 30% less to be precise.

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